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Category:SolidWorks Category:Computer-aided design softwareCloning of human cyclophilin B: tissue expression and inhibition of papain activity by cyclophilin B and isosorbide 5-mononitrate. Cyclophilin B (CyPB), a member of the immunophilin family, is one of the major inhibitors of proteinase-catalyzed protein folding. The present study examined the distribution of CyPB mRNA in human tissues, and the effect of isosorbide 5-mononitrate (IS-5-MNT), a CyPB inhibitor, on human papain (H-PAP) activity. CyPB mRNA was detected by RT-PCR analysis of total RNA extracted from the human tissues. H-PAP activity was inhibited by IS-5-MNT in a dose-dependent manner. This study is the first report to characterize the presence of CyPB in the human tissues.
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